Journal: Cell Reports

Article Title: Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells

doi: 10.1016/j.celrep.2020.107894

Figure Lengend Snippet: Activation and Killing by T Cells Is Selective for iPSC-β (A) Experimental design: PBMCs co-cultured with autologous iPSC-derived cells. (B, C, and F) CD25 or CD69 expression shown as MFI. (B) PBMCs (gated on CD3 + , CD4 + , and CD8 + populations) co-cultured for 48 h with autologous iPSC-β (purple) or iPSC-α (red). (n = 3 T1D and n = 1 ND donor, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (C) Donor-matched PBMCs (CD3 + gated) (n = 1 T1D, n = 3 differentiation batches per donor line) co-cultured for 48 h with autologous iPSC-β (purple) or iPSC-cardiomyocytes (orange). (D) Percentage of live iPSC-β (C-peptide + /glucagon − ) or iPSC-α (C-peptide − /glucagon + ) from iPSC-β or iPSC-α differentiations, respectively (n = 3 T1D donors, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (F) Representative flow cytometry histograms after 48 h of co-culture. Dashed histogram represents the control (untreated target cells). (E) Percentage of apoptotic (apopxin + ) iPSC-β (CD49a + /CD26 − ) or iPSC-α (CD49a − /CD26 + ) from iPSC-β or iPSC-α differentiations (n = 1 T1D iPSC donor, n = 3 differentiation batches per donor line). (G) Unmatched PBMCs (CD3 + gated cells) co-cultured for 48 h with iPSC-α (n = 3 T1D donors, n = 3 differentiation batches per donor line). T1D 1 , T1D 2 , and T1D 3 were pooled together. (H) Donor-matched PBMCs (gated on CD3 + , CD4 + , and CD8 + populations) co-cultured for 48 h with autologous enriched iPSC-β or iPSC-α. Data are means ± SEMs, 2-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, and ∗∗∗∗ p < 0.0001; ns, non-significant. See also Figure S4 .

Article Snippet: For the apoptosis assay, clusters were dissociated, and single cells were stained at room temperature for 30 min using a 1:100 dilution of recently reported stem cell derived β-cell marker, anti-human CD49a ( ) PE-conjugated (BD Biosciences, 559596), α-cell marker anti-human CD26 APC-conjugated (Miltenyi Biotech, 130-120-769), and Apopxin green indicator (Abcam, ab176750).

Techniques: Activation Assay, Cell Culture, Derivative Assay, Expressing, Flow Cytometry, Co-Culture Assay, Control

Journal: Cell Reports

Article Title: Modeling Type 1 Diabetes In Vitro Using Human Pluripotent Stem Cells

doi: 10.1016/j.celrep.2020.107894

Figure Lengend Snippet:

Article Snippet: For the apoptosis assay, clusters were dissociated, and single cells were stained at room temperature for 30 min using a 1:100 dilution of recently reported stem cell derived β-cell marker, anti-human CD49a ( ) PE-conjugated (BD Biosciences, 559596), α-cell marker anti-human CD26 APC-conjugated (Miltenyi Biotech, 130-120-769), and Apopxin green indicator (Abcam, ab176750).

Techniques: Virus, Recombinant, Software, Real-time Polymerase Chain Reaction

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Double immunofluorescent staining of human subcutaneous adipose tissue sections with antibodies against T-cadherin (green) and DPP4 (red); nuclei were counterstained with DAPI (blue). Images were acquired using a Zeiss LSM 780 confocal microscope and ZEN2010 software, shown at lower magnification (A) and higher magnification (B) . A thick arrow points to a group of cells expressing both T-cadherin and DPP4 in the interstitium; thin arrows mark cells expressing only T-cadherin; ovals encircle adipocytes. Scale bar 50 µm. (C) The table shows the percentage of T-cadherin–positive, DPP4 + cells and double-positive cells (DPP4 + /T-cadherin + ), quantified from adipose tissue sections of two healthy donors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Staining, Microscopy, Software, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Light microscopy of MSCs (of the two to three passages) isolated from human subcutaneous adipose tissue of a healthy donor (A) and immunofluorescent staining with antibodies against T-cadherin (green) (B) . Arrows indicate cells with low or no T-cadherin expression, whereas cells exhibiting green fluorescence corresponding to T-cadherin are clearly visible. Scale bar, 50 µm. Light microscopy of human MSCs (C) and double immunofluorescent staining with antibodies against T-cadherin green, (E) and DPP4 red, (F) nuclei were counterstained with DAPI blue, (D) . Arrows in (C–F) indicate one and the same cell co-expressing T-cadherin and DPP4. Images were acquired using a Leica DMI 6000B microscope equipped with a Leica DFC7000T digital camera and LAS X software. Scale bar, 20 µm. (G) Representative flow cytometry plot showing T-cadherin and DPP4 distribution in cultured MSCs. The proportion of double-positive (DPP4 + /T-cadherin + ) cells was 30.4%; 6.15% expressed only T-cadherin, and 14% expressed only DPP4.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Light Microscopy, Isolation, Staining, Expressing, Fluorescence, Microscopy, Software, Flow Cytometry, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Individual UMAP plots showing the expression levels and distribution of CDH13 (encoding T-cadherin) in control MSCs (A) and MSCs after 4 days of adipogenic induction (B) . UMAP plots demonstrating DPP4 expression in control MSCs (C) and MSCs after 4 days of adipogenic induction (D) . (E) RT-qPCR analysis of MSCs cultured in control medium or under adipogenic induction conditions showing the dynamics of T-cadherin mRNA expression. T-cadherin/ CDH13 expression decreased by day 4 in adipogenic medium and remained low through day 10. RT-qPCR data are shown as the mean ± SD. T-test. **р< 0.01 *p < 0.05 vs. control media in corresponding experimental day. Results are representative of three biologically independent experiments.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Quantitative RT-PCR, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. (A) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression (encoding for T-cadherin) in the integrated object; CDH13 expressing cells corresponds to Cluster 3 (more than 1-fold change of the average expression level); (B) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the integrated object; DPP4 expressing cells correspond to Cluster 3 (more than 1-fold change of the average expression level) (C) DimPlot–Integrated object UMAP-clustering. Sample proportion diagrams depict the ratio between the cell counts in the control MSC sample (Salmon) and in the MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation within the Clusters. (D) DimPlot–Integrated object grouped by samples. CDH13 expression in the control MSC sample (Salmon) and MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation. Cluster 3 predominantly contains cells from the control sample.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. FeaturePlot. Each cluster is denoted by color. Cluster 0 (Salmon) primarily contains cells expressing fibroblast markers and genes responsible for cell cycle regulation. Cluster 1 (Khaki) encompasses cells expressing preadipocyte-specific genes, such as CEBPB , PPARγ, CD36 and markers of mature adipocytes ( ADIPOQ , Perilipin1 , Perilipin4 ). In Cluster 2 (green), cells predominantly express genes related to mitosis. Cluster 3 (Blue) contains cells of interest with high level of T-cadherin expression, as well as classical MSC markers ( CD90 , PDGFR ), Wnt signaling genes , and DPP4 . In a separate remote Cluster 4 (Magenta), besides CDH13 , cells express Nestin , a marker of neural crest cells, and CD36 , a marker of adipocyte progenitors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Split violin-plots showing the relative expression levels and distribution of CDH13 (A) and DPP4 (B) genes in the control MSC sample (Salmon) and MSC sample after a 4-day induction of adipogenic differentiation (Iris blue). The highest CDH13 expression was detected in Cluster 3 in MSCs of the control sample compared to MSCs after a 4-day adipogenic induction. Similarly, the highest expression of DPP4 was found in Cluster 3 in MSCs of the control sample. Split violin plots were generated using the R package Seurat and the function VlnPlot with the argument split.by = “sample”.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Generated

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: (A) DimPlot– GSE182158 object UMAP-clustering; (B) 2 cluster manual cell type annotation, the red oval marks cluster 2; (C) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression in the GSE182158 object; (D) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the GSE182158 object.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Elevated DPP4 expression in MSCs after lentiviral transduction in T-cadherin-overexpressing cells was verified using RT-qPCR (A) and Western blot (B) . β-tubulin was used as the loading control for Western blot analysis. Representative results from one of two biologically independent RT-qPCR and eight Western blot experiments are shown. ANOVA with multiple comparisons, **p < 0.01.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Transduction, Quantitative RT-PCR, Western Blot, Control

Journal: International Journal of Molecular Medicine

Article Title: Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis

doi: 10.3892/ijmm.2025.5592

Figure Lengend Snippet: Serial changes of serum levels of BUN, creatinine, DPP4 and GLP-1 and R Up/c . Circulating levels of (A) BUN, (B) creatinine, (C) DPP4 and (D) GLP-1, and (E) R Up/c at baseline. Circulating levels of (F) BUN, (G) creatinine, (H) DPP4 and (I) GLP-1, and (J) R Up/c at day 14 after CKD induction. Circulating levels of (K) BUN, (L) creatinine, (M) DPP4 and (N) GLP-1, and (O) R Up/c at day 35 after CKD induction. Circulating levels of (P) BUN, (Q) creatinine, (R) DPP4 and (S) GLP-1, and (T) R Up/c at day 42 after CKD induction. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni's multiple comparisons post hoc test (n=6/group). # P<0.05, ## P<0.01 vs. SC; * P<0.05 vs. CKD; ns, not significant. BUN, blood urea nitrogen; CG, chlorhexidine gluconate; CKD, chronic kidney disease; DPP4, dipeptidyl peptidase 4; GLP-1, glucagon-like peptide 1; R uP/uC , ratio of urine protein/urine creatinine; SC, sham control.

Article Snippet: Primary antibodies used in the western blot analysis included: NOX-1 (1:1,000; cat. no. SAB4200097; Sigma-Aldrich; Merck KGaA), NOX-2 (1:1,000; cat. no. MABS2195; Sigma-Aldrich; Merck KGaA), TNF-α (1:1,000; cat. no. 3707; Cell Signaling Technology, Inc.), IL-1α (1:1,000; cat. no. 84618; Cell Signaling Technology, Inc.), MMP-9 (1:1,000; cat. no. ab76003; Abcam), DPP4 (1:1,000; cat. no. ARP63319_P050; Aviva Systems Biology), phosphorylated (p)-Smad3 (1:1,000; cat. no. 9520; Cell Signaling Technology, Inc.), Smad3 (1:1,000; cat. no. 9513; Cell Signaling Technology, Inc.), TGF-β (1:3,000; cat. no. ab215715; Abcam), GLP-1 (1:1,000; cat. no. ab108443; Abcam), GLP-1R (1:1,000; cat. no. ab218532; Abcam), nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000; cat. no. ab62352; Abcam), NAD(P)H quinone oxidoreductase 1 (NQO-1; 1:1,000; cat. no. ab80588; Abcam), Snail (1:1,000; cat. no. 3879; Cell Signaling Technology, Inc.), von Willebrand factor (vWF; 1:1,000; cat. no. ab154193; Abcam), VEGF (1:1,000; cat. no. ab214424; Abcam), α-smooth muscle actin (α-SMA; 1:6,000; cat. no. A2547; Sigma-Aldrich; Merck KGaA), vimentin (1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), β-catenin (1:1,000; cat. no. 8480; Cell Signaling Technology, Inc.), fibronectin (1:1,000 cat. no. ab2413; Abcam), collagen I (1:1,000; cat. no. C2456; Sigma-Aldrich; Merck KGaA), N-cadherin (1:1,000; cat. no. 13116; Cell signaling Technology, Inc.), TLR-2 (1:4,000; cat. no. ab213676; Abcam), TLR-4 (1:4,000; cat. no. NB100-56566; Novus Biologicals, LLC; Bio-Techne), NF-κB (1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p-NF-κB (1:1,000; cat. no. 3033; Cell Signaling Technology, Inc.), CD31 (1:1,000; cat. no. 77699; Cell Signaling Technology, Inc.) and β-actin (1:10,000; cat. no. A5441; MilliporeSigma).

Techniques: Control

Journal: International Journal of Molecular Medicine

Article Title: Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis

doi: 10.3892/ijmm.2025.5592

Figure Lengend Snippet: Protein expression levels of biomarkers of oxidative stress, inflammation and angiogenesis, as well as DPP4, GLP-1R and antioxidants in the peritonium by day 42 after CKD induction. (A) Protein expression levels were detected by western blot analysis. Semi-quantification of (B) NOX-1, (C) vWF, (D) GLP-1R, (E) NOX-2, (F) TNF-α, (G) Nrf2, (H) NQO-1, (I) p-NF-κB/NF-κB, (J) CD31, (K) DPP4 and (L) VEGF. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni's multiple comparisons post hoc test (n=6/group). # P<0.05, ## P<0.01 vs. SC; * P<0.05 vs. CKD. CG, chlorhexidine gluconate; CKD, chronic kidney disease; DPP4, dipeptidyl peptidase 4; GLP-1R, glucagon-like peptide 1 receptor; NQO-1, NAD(P)H-quinone oxidoreduc-tase 1; Nrf2, nuclear factor erythroid 2-related factor 2; p-, phosphorylated; SC, sham control; vWF, von Willebrand factor.

Article Snippet: Primary antibodies used in the western blot analysis included: NOX-1 (1:1,000; cat. no. SAB4200097; Sigma-Aldrich; Merck KGaA), NOX-2 (1:1,000; cat. no. MABS2195; Sigma-Aldrich; Merck KGaA), TNF-α (1:1,000; cat. no. 3707; Cell Signaling Technology, Inc.), IL-1α (1:1,000; cat. no. 84618; Cell Signaling Technology, Inc.), MMP-9 (1:1,000; cat. no. ab76003; Abcam), DPP4 (1:1,000; cat. no. ARP63319_P050; Aviva Systems Biology), phosphorylated (p)-Smad3 (1:1,000; cat. no. 9520; Cell Signaling Technology, Inc.), Smad3 (1:1,000; cat. no. 9513; Cell Signaling Technology, Inc.), TGF-β (1:3,000; cat. no. ab215715; Abcam), GLP-1 (1:1,000; cat. no. ab108443; Abcam), GLP-1R (1:1,000; cat. no. ab218532; Abcam), nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000; cat. no. ab62352; Abcam), NAD(P)H quinone oxidoreductase 1 (NQO-1; 1:1,000; cat. no. ab80588; Abcam), Snail (1:1,000; cat. no. 3879; Cell Signaling Technology, Inc.), von Willebrand factor (vWF; 1:1,000; cat. no. ab154193; Abcam), VEGF (1:1,000; cat. no. ab214424; Abcam), α-smooth muscle actin (α-SMA; 1:6,000; cat. no. A2547; Sigma-Aldrich; Merck KGaA), vimentin (1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), β-catenin (1:1,000; cat. no. 8480; Cell Signaling Technology, Inc.), fibronectin (1:1,000 cat. no. ab2413; Abcam), collagen I (1:1,000; cat. no. C2456; Sigma-Aldrich; Merck KGaA), N-cadherin (1:1,000; cat. no. 13116; Cell signaling Technology, Inc.), TLR-2 (1:4,000; cat. no. ab213676; Abcam), TLR-4 (1:4,000; cat. no. NB100-56566; Novus Biologicals, LLC; Bio-Techne), NF-κB (1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p-NF-κB (1:1,000; cat. no. 3033; Cell Signaling Technology, Inc.), CD31 (1:1,000; cat. no. 77699; Cell Signaling Technology, Inc.) and β-actin (1:10,000; cat. no. A5441; MilliporeSigma).

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Molecular Medicine

Article Title: Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis

doi: 10.3892/ijmm.2025.5592

Figure Lengend Snippet: LPS induces peritoneal damaged by day 5 of treatment. Circulating levels of (A) GLP-1 and (B) DPP4 at baseline. Circulating levels of (C) GLP-1 and (D) DPP4 at day 5. Abdominal levels of (E) GLP-1 and (F) DPP4 at day 5. Circulating levels of the indicator of peritoneal permeability FITC-dextran (G) 10, (H) 20 and (I) 30 min after LPS treatment. Flow cytometric analysis of the number of (J) CD11b/c + , (K) MPO + anf (L) Ly6G + cells in circulation. Flow cytometric analysis of the number of (M) CD11b/c + , (N) MPO + cells and (O) Ly6G + cells in the abdominal fluid. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni's multiple comparisons post hoc test (n=6/group). # P<0.05 vs. SC; * P<0.05 vs. LPS. DPP4, dipeptidyl peptidase 4; GLP-1, glucagon-like peptide 1; LPS, lipopolysaccharide; MPO, myeloperoxidase; SC, sham control.

Article Snippet: Primary antibodies used in the western blot analysis included: NOX-1 (1:1,000; cat. no. SAB4200097; Sigma-Aldrich; Merck KGaA), NOX-2 (1:1,000; cat. no. MABS2195; Sigma-Aldrich; Merck KGaA), TNF-α (1:1,000; cat. no. 3707; Cell Signaling Technology, Inc.), IL-1α (1:1,000; cat. no. 84618; Cell Signaling Technology, Inc.), MMP-9 (1:1,000; cat. no. ab76003; Abcam), DPP4 (1:1,000; cat. no. ARP63319_P050; Aviva Systems Biology), phosphorylated (p)-Smad3 (1:1,000; cat. no. 9520; Cell Signaling Technology, Inc.), Smad3 (1:1,000; cat. no. 9513; Cell Signaling Technology, Inc.), TGF-β (1:3,000; cat. no. ab215715; Abcam), GLP-1 (1:1,000; cat. no. ab108443; Abcam), GLP-1R (1:1,000; cat. no. ab218532; Abcam), nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000; cat. no. ab62352; Abcam), NAD(P)H quinone oxidoreductase 1 (NQO-1; 1:1,000; cat. no. ab80588; Abcam), Snail (1:1,000; cat. no. 3879; Cell Signaling Technology, Inc.), von Willebrand factor (vWF; 1:1,000; cat. no. ab154193; Abcam), VEGF (1:1,000; cat. no. ab214424; Abcam), α-smooth muscle actin (α-SMA; 1:6,000; cat. no. A2547; Sigma-Aldrich; Merck KGaA), vimentin (1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), β-catenin (1:1,000; cat. no. 8480; Cell Signaling Technology, Inc.), fibronectin (1:1,000 cat. no. ab2413; Abcam), collagen I (1:1,000; cat. no. C2456; Sigma-Aldrich; Merck KGaA), N-cadherin (1:1,000; cat. no. 13116; Cell signaling Technology, Inc.), TLR-2 (1:4,000; cat. no. ab213676; Abcam), TLR-4 (1:4,000; cat. no. NB100-56566; Novus Biologicals, LLC; Bio-Techne), NF-κB (1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p-NF-κB (1:1,000; cat. no. 3033; Cell Signaling Technology, Inc.), CD31 (1:1,000; cat. no. 77699; Cell Signaling Technology, Inc.) and β-actin (1:10,000; cat. no. A5441; MilliporeSigma).

Techniques: Permeability, Control

Journal: International Journal of Molecular Medicine

Article Title: Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis

doi: 10.3892/ijmm.2025.5592

Figure Lengend Snippet: Schematic diagram of the proposed underlying mechanims of dulaglutide treatment of peritoneal fibrosis and PD failure. CKD, chronic kidney disease; DPP4, dipeptidyl peptidase 4; EMT, epithelial-mesenchymal transion; GLP-1, glucagon-like peptide 1; GLP-1R, GLP-1 receptor; LPS, lipopolysac-charide; NQO-1, NAD(P)H-quinone oxidoreductase 1; Nrf2, nuclear factor erythroid 2-related factor 2; PD, pertoneal dialysis.

Article Snippet: Primary antibodies used in the western blot analysis included: NOX-1 (1:1,000; cat. no. SAB4200097; Sigma-Aldrich; Merck KGaA), NOX-2 (1:1,000; cat. no. MABS2195; Sigma-Aldrich; Merck KGaA), TNF-α (1:1,000; cat. no. 3707; Cell Signaling Technology, Inc.), IL-1α (1:1,000; cat. no. 84618; Cell Signaling Technology, Inc.), MMP-9 (1:1,000; cat. no. ab76003; Abcam), DPP4 (1:1,000; cat. no. ARP63319_P050; Aviva Systems Biology), phosphorylated (p)-Smad3 (1:1,000; cat. no. 9520; Cell Signaling Technology, Inc.), Smad3 (1:1,000; cat. no. 9513; Cell Signaling Technology, Inc.), TGF-β (1:3,000; cat. no. ab215715; Abcam), GLP-1 (1:1,000; cat. no. ab108443; Abcam), GLP-1R (1:1,000; cat. no. ab218532; Abcam), nuclear factor erythroid 2-related factor 2 (Nrf2; 1:1,000; cat. no. ab62352; Abcam), NAD(P)H quinone oxidoreductase 1 (NQO-1; 1:1,000; cat. no. ab80588; Abcam), Snail (1:1,000; cat. no. 3879; Cell Signaling Technology, Inc.), von Willebrand factor (vWF; 1:1,000; cat. no. ab154193; Abcam), VEGF (1:1,000; cat. no. ab214424; Abcam), α-smooth muscle actin (α-SMA; 1:6,000; cat. no. A2547; Sigma-Aldrich; Merck KGaA), vimentin (1:1,000; cat. no. 5741; Cell Signaling Technology, Inc.), β-catenin (1:1,000; cat. no. 8480; Cell Signaling Technology, Inc.), fibronectin (1:1,000 cat. no. ab2413; Abcam), collagen I (1:1,000; cat. no. C2456; Sigma-Aldrich; Merck KGaA), N-cadherin (1:1,000; cat. no. 13116; Cell signaling Technology, Inc.), TLR-2 (1:4,000; cat. no. ab213676; Abcam), TLR-4 (1:4,000; cat. no. NB100-56566; Novus Biologicals, LLC; Bio-Techne), NF-κB (1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), p-NF-κB (1:1,000; cat. no. 3033; Cell Signaling Technology, Inc.), CD31 (1:1,000; cat. no. 77699; Cell Signaling Technology, Inc.) and β-actin (1:10,000; cat. no. A5441; MilliporeSigma).

Techniques:

Journal: The Journal of Infectious Diseases

Article Title: Middle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways

doi: 10.1093/infdis/jiv380

Figure Lengend Snippet: Middle East respiratory syndrome coronavirus (MERS-CoV) differentially infects subsets of human peripheral blood mononuclear cells (PBMCs). A , Human PBMCs were infected with MERS-CoV at 2 50% tissue culture infective doses per cell. At 24 hours after infection, infected cells were fixed with 4% paraformaldehyde (PA) and immunolabeled for detection of cell surface markers and MERS-CoV nucleoprotein (NP). The shaded curve and the solid line represent MERS-CoV NP expression from mock-infected and MERS-CoV–infected cells, respectively. The summary panel at the right represents the average percentage of infected cells from 3 different donors. B , Uninfected human PBMCs were fixed with 4% PA and immunolabeled for detection of surface DPP4 expression. The shaded curve and the solid line represented isotype and DPP4-specific staining, respectively. The summary panel at the right represents the average mean fluorescent intensity (MFI) from 3 different donors. In all panels, bars and error bars represented means and standard deviations, respectively. Statistical analyses were performed using the Student t test. * P < .001. Abbreviation: NK, natural killer.

Article Snippet: DPP4 antibody was obtained from R&D Systems.

Techniques: Infection, Immunolabeling, Expressing, Staining

Journal: The Journal of Infectious Diseases

Article Title: Middle East Respiratory Syndrome Coronavirus Efficiently Infects Human Primary T Lymphocytes and Activates the Extrinsic and Intrinsic Apoptosis Pathways

doi: 10.1093/infdis/jiv380

Figure Lengend Snippet: Middle East respiratory syndrome coronavirus (MERS-CoV) efficiently infects CD4 + and CD8 + T cells and downregulates surface dipeptidyl peptidase 4 (DPP4) in the infected cells. A , T cells were infected with MERS-CoV and severe acute respiratory syndrome CoV (SARS-CoV) at 2 50% tissue culture infective doses (TCID 50 ) per cell. Cells were fixed at 24 hours after infection and immunolabeled for detection of expression of MERS-CoV NP or SARS-CoV NP. The shaded curve and the solid line represent virus NP expression from mock-infected and MERS/SARS-CoV–infected cells, respectively. B , T cells were infected with MERS-CoV at 2 TCID 50 per cell. Cells were fixed at 24 hours after infection and immunolabeled for detection of CD4 or CD8 and MERS-CoV NP expression. The shaded curve and the solid line represent NP expression from mock-infected and MERS-CoV–infected cells, respectively. C , Uninfected T cells were fixed and immunolabeled for detection of CD4 or CD8 and surface DPP4 expression. The shaded curve and the solid line represent isotype and DPP4-specific staining, respectively. D , T cells were infected with MERS-CoV at 2 TCID 50 per cell. Cells were fixed at 24 hours after infection and immunolabeled for detection of CD4 or CD8, DPP4, and MERS-CoV NP expression. Surface or total DPP4 was detected by labeling the cells with the DPP4 antibody before or after cell permeabilization, respectively. The shaded curve represents isotype staining of DPP4. The solid line and dotted line represent DPP4 staining from mock-infected and MERS-CoV–infected cells, respectively. The DPP4 mean fluorescence intensity (MFI) in infected cells was calculated on the basis of CD4 + /MERS-CoV NP–expressing or CD8 + /MERS-CoV NP–expressing double-positive T-cell data. The DPP4 MFI in mock-infected cells was calculated on the basis of CD4 + or CD8 + T-cell data. The summary panels at the right represent the average percentage of infected cells ( A and B ), MFI ( C ), or percentage of mock MFI ( D ) from 3 different donors. In all panels, bars and error bars represent means and standard deviations. Statistical analyses were performed using the Student t test. * P < .05.

Article Snippet: DPP4 antibody was obtained from R&D Systems.

Techniques: Infection, Immunolabeling, Expressing, Virus, Staining, Labeling, Fluorescence

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Panel for flow cytometry analysis of sorted populations

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Flow Cytometry, Marker, Staining

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Sample detail for fluorescence compensation settings

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Fluorescence, Staining, Suspension

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Flow cytometry gating and isolated fraction purity/viability (A) Gating strategy for cellular subsets identification. Singlet live cells were obtained by using SSC/Time, FSC/SSC and FSC/mortality dye (LD) parameters. (B) To appreciate each fraction purity, dot plot with CD45, CD31, Lin and CD26 (CAFs marker) staining were shown in CD45+ TILs, tumor cells and CAFs in total tumor suspension. (C) Proportion of CD45+ TILs, Endothelial cells, Tumor cells and CAFs in tumor cell suspension and purity of each cell subsets in isolated fractions.

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Flow Cytometry, Isolation, Marker, Staining, Suspension

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet: Phenotypic and transcriptomic analysis (A and B) Representative flow cytometry analysis of CAFs markers (CD26, FAPa, PDPN, PDGFRa, PDGFRb) on tumor cells and CAFs fractions (A). Normalized expression of each marker (B). (C) Analysis of CAFs, CD45+ TILs and tumor associated genes expression by RT-qPCR in each isolated fraction (n = 3 samples/fraction).

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Flow Cytometry, Expressing, Marker, Quantitative RT-PCR, Isolation

Journal: STAR Protocols

Article Title: Simultaneous isolation of CD45 tumor-infiltrating lymphocytes, tumor cells, and associated fibroblasts from murine breast tumor model by MACS

doi: 10.1016/j.xpro.2022.101951

Figure Lengend Snippet:

Article Snippet: CD26 Antibody, anti-mouse, REAfinity- PE (1:100 dilution) , Miltenyi Biotec , Cat# 130-122-775, RRID: AB_2801934.

Techniques: Recombinant, Flow Cytometry, Staining, Isolation, Cell Isolation, Software, Real-time Polymerase Chain Reaction, Blocking Assay, Gentle