Journal: Emerging Infectious Diseases

Article Title: Domestic Pig Unlikely Reservoir for MERS-CoV

doi: 10.3201/eid2306.170096

Figure Lengend Snippet: Comparison of the amino acid residues shown to be essential in binding of Middle East respiratory syndrome coronavirus spike protein to DPP4 of human, dromedary camel, and domestic pig*

Article Snippet: We investigated whether DPP4 is expressed in the pig respiratory tract by performing immunohistochemical staining on the nasal mucosa and lung tissue obtained from healthy pigs using an antibody against DPP4 (mouse monoclonal anti-DPP4 [CD26], clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA).

Techniques: Comparison, Binding Assay

Journal: Emerging Infectious Diseases

Article Title: Domestic Pig Unlikely Reservoir for MERS-CoV

doi: 10.3201/eid2306.170096

Figure Lengend Snippet: Dipeptidyl peptidase (DPP) 4 expression in the domestic pig respiratory tract. Tissues were stained by using a cross-reactive mouse monoclonal antibody against DPP4 (CD26, clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA). DPP4 expression was absent in the nasal mucosa (A) but present in lung tissue (B) of healthy domestic pigs. Original magnification: nasal mucosa ×40; lung ×200.

Article Snippet: We investigated whether DPP4 is expressed in the pig respiratory tract by performing immunohistochemical staining on the nasal mucosa and lung tissue obtained from healthy pigs using an antibody against DPP4 (mouse monoclonal anti-DPP4 [CD26], clone OTI11D7, 1:2,500; Origene Technologies, Inc., Rockville, MD, USA).

Techniques: Expressing, Staining

Journal: Hypertension

Article Title: Dipeptidyl Peptidase IV Regulates Proliferation of Preglomerular Vascular Smooth Muscle and Mesangial Cells

doi: 10.1161/hypertensionaha.112.196501

Figure Lengend Snippet: Figure 1. A, Dipeptidyl peptidase IV (DPPIV) mRNA levels in spontaneously hypertensive rat (SHR) and Wistar-Kyoto rat (WKY) preglomerular microvascular smooth muscle cells (PGVSMCs) and glomerular mesangial cells (GMCs). DPPIV mRNA expression was determined by quantitative real-time PCR and normalized to -actin mRNA. B, Western blotting for DPPIV protein using 2 different antibodies (Ab 1 and 2). A dis- tinct and single band was observed at 55 kDa for both WKY and SHR PGVSMCs and GMCs. C, DPPIV protein expression in SHR and WKY PGVSMCs and GMCs. DPPIV protein expression was quantified by densitometry analysis of Western blots and normalized to -actin protein. Letter above bars (a) indicates significantly different (Fisher least significant difference [LSD] test) from PGVSMCs.

Article Snippet: To ensure that the signal detected by Western blotting was indeed DPPIV, we performed Western blotting for DPPIV protein as described in Jackson et al.3 using two different primary antibodies: 1) rabbit polyclonal anti-DPPIV antibody (Proteintech Group, Inc.; Chicago, IL; catalog No. 10940-1-AP; 1:600); and 2) rabbit polyclonal anti-DPPIV antibody (Thermo Scientific, Rockford, IL; catalogue number PA1-8455; 1:2500).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet: Combination of CITE-seq, scRNA-seq, snRNA-seq, and spatial analyses enables identification of all hepatic cell types including bona fide cell doublets, related to Figure 1 (A and B) Top DEGs (A) and DEPs (B) for cell types from Figure 1 B. (C) Distinct profiles of cells or nuclei within the UMAP depending on isolation protocols; 71,162 cells from ex vivo digestions, 96,066 cells from in vivo digestions, and 18,666 nuclei. Numbers on plots represent numbers of cells/nuclei per population. (D) Correlation plots showing genes captured within the KC, B cell and neutrophil populations with and without addition of CITE-seq antibodies. (E) Expression of VSIG4, CD206, and ESAM (protein, top) and Vsig4 , Mrc1 , and Esam (mRNA, bottom). (F) UMAP showing clusters of cells when only minimal QC for gene number and % mitochondrial genes is performed; 17,669 cells pooled from 3 samples. Expression of Cd5l, Cd19 , and Kdr by the clusters facilitating identification of cell types per annotation. (G) CITE-seq data from (F) in Flow-Jo showing expression of CD206 and ESAM in total KCs (left) and total B cells (middle). Numbers represent % of entire KC or B cell population. Identified populations were then mapped back onto the original UMAP (right). (H) Expression of CD31, CD26, and CD38 by indicated populations. (I) Heatmaps showing expression of top DEGs between KC1s and LSECs (left), KC2s and KC1s + LSECs (middle) and B cell2s and B cell1s + LSECs (right). (J) 3D reconstruction of murine liver following perfusion with antigen fix to inflate endothelial cells and staining with antibodies against CD31, CD206, and F4/80. (K) UMAP showing clusters generated from Visium analysis of liver tissue (4 samples) and liver capsule (1 sample). (L) Top unbiased genes defining zonation trajectory from portal to central vein in Visium. (M) Expression of Glul and Epcam by confocal microscopy (left), annotation of portal, periportal, mid, and central regions on same tissue section (middle) and overlay of both datasets (right). (N) Identification of cholangiocyte (left) and cDC (right) signatures on zonated Visium spots. (P) Molecular Cartography showing expression of indicated zonated hepatocyte mRNAs in liver tissue. Data are representative of 2 mice. (O) Expression of Itgae (encoding CD103) in the UMAP of the total liver (left) and flow cytometric analysis of total cDC1s for CD103 and MHCII expression in the healthy murine liver (right).

Article Snippet: Anti-Mouse CD26 (REA1196) PE , Miltenyi Biotec , 130-122-775; RRID: AB_2801934.

Techniques: Isolation, Ex Vivo, In Vivo, Expressing, Staining, Generated, Confocal Microscopy

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD26 (REA1196) PE , Miltenyi Biotec , 130-122-775; RRID: AB_2801934.

Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy

Journal: Oncology reports

Article Title: Upregulated expression and activation of membrane‑associated proteases in esophageal squamous cell carcinoma.

doi: 10.3892/or.2014.3162

Figure Lengend Snippet: Figure 1. Expression of FAP-α and DPPIV in paired tumor and normal esophageal tissues. (Upper panels) Four representative examples of FAP-α (544-bp amplification product) and DPPIV (473-bp amplification product) mRNA expression as determined using RT-PCR in cancer (ESCC) and non‑cancer (ENCC) samples. The expression of the actin transcripts (479 bp) is shown as a positive control. I-IV, patient codes. (Bottom panels) Semi‑quantitative analysis of the FAP-α and DPPIV PCR products of the control and neoplastic samples. The values are normalized to β-actin. The results are presented as the ratio of cancer to normal tissue. The bar chart shows mean values and standard error (left). The scatter chart with separate measurements shows the range of calculated values (right). Statistically significant differences between the levels of amplification of both FAP-α and DPPIV cDNA in the cancer and non-cancer tissues were found. ENCC, esophageal non-cancer tissues from areas distant to the tumor. ESCC, esophageal squamous cell carcinoma tissues in the form of paired specimens of malignant tumors. FAP-α, fibroblast activation protein-α; DPPIV, dipeptidyl peptidase IV.

Article Snippet: The antibodies used were: DPP4 antibody-C-terminal region rabbit polyclonal antibody (ARP63319_P050; Aviva Systems Biology); MT-MMP-1 (H-72) rabbit polyclonal antibody (sc-30074); FAP-α (C-19) goat polyclonal antibody (sc-54539) (both from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and seprase rabbit polyclonal antibody (N1N3) (GTX102732; GeneTex, Inc.).

Techniques: Expressing, Amplification, Reverse Transcription Polymerase Chain Reaction, Positive Control, Control, Activation Assay

Journal: Oncology reports

Article Title: Upregulated expression and activation of membrane‑associated proteases in esophageal squamous cell carcinoma.

doi: 10.3892/or.2014.3162

Figure Lengend Snippet: Figure 2. FAP-α and DPPIV protein levels in tumor and normal esophageal tissues. (Upper panels) Examples of FAP-α western blot immunostaining of four paired non-cancer (ENCC) and cancer (ESCC) samples. The bands indicate a 97-kDa full-length monomeric form in three cancer tissues and a 55-kDa proteolytic fragment in all of the non-cancer and cancer samples. Immunodetection of β-actin, a 43-kDa protein, was used for normalization. I-IV, patient codes. (Bottom panels) Semi-quantitative analysis of DPPIV in the control and neoplastic samples. Post-normalization data are presented as the ratio of cancer to normal tissue. The bar chart shows the mean values and standard error (left). The scatter chart with separate measurements shows the range of calculated values (right). Statistically significant differences were found between the level of DPPIV protein in the cancerous and non-cancerous tissues. FAP-α, fibroblast activation protein-α; DPPIV, dipeptidyl peptidase IV; ESCC, esophageal squamous cell carcinoma.

Article Snippet: The antibodies used were: DPP4 antibody-C-terminal region rabbit polyclonal antibody (ARP63319_P050; Aviva Systems Biology); MT-MMP-1 (H-72) rabbit polyclonal antibody (sc-30074); FAP-α (C-19) goat polyclonal antibody (sc-54539) (both from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and seprase rabbit polyclonal antibody (N1N3) (GTX102732; GeneTex, Inc.).

Techniques: Western Blot, Immunostaining, Immunodetection, Control, Activation Assay

Journal: The American Journal of Pathology

Article Title: Clinicopathologic, Immunohistochemical, and Ultrastructural Findings of a Fatal Case of Middle East Respiratory Syndrome Coronavirus Infection in the United Arab Emirates, April 2014

doi: 10.1016/j.ajpath.2015.10.024

Figure Lengend Snippet: Primary Antibodies Used for IHC

Article Snippet: 1547 , DPP4 (clone OTI11D7) , MAB , 1:100 , PK , Origene Technologies , (no. 1371) Envision Doublestain , .

Techniques:

Journal: The American Journal of Pathology

Article Title: Clinicopathologic, Immunohistochemical, and Ultrastructural Findings of a Fatal Case of Middle East Respiratory Syndrome Coronavirus Infection in the United Arab Emirates, April 2014

doi: 10.1016/j.ajpath.2015.10.024

Figure Lengend Snippet: Immunohistochemical and ultrastructural localization of MERS-CoV and associated histologic findings. A: MERS-CoV and cytokeratin antigens in pneumocytes ( arrow ); red stain, MERS-CoV; brown stain, cytokeratin. B: MERS-CoV antigens in pneumocytes ( arrowhead ) and CD68 antigens in macrophages ( arrow ); red stain, CD68; brown stain, MERS-CoV. C: MERS-CoV and surfactant antigens in type 2 pneumocytes ( arrow ); red stain, surfactant; brown stain, MERS-CoV. D: MERS-CoV and DPP4 in pneumocytes ( arrow ); red stain, DPP4; brown stain, MERS-CoV. E: Fragmented pneumocyte infected with MERS-CoV, hyaline membrane ( arrowhead ) present. F: Magnified from the boxed area in E . MERS-CoV virions dispersed as single particles ( arrow ) or in clusters within membrane-bound vesicles ( arrowhead ). Spherical and pleomorphic particles ranged in size from 50 to 150 nm diameter. Scale bars: 2 μm ( E ); 500 nm ( F ). Original magnification: ×100 ( A and C ); ×63 ( B ); ×75 ( D ). DPP4, dipeptidyl peptidase 4; MERS-CoV, Middle East respiratory syndrome coronavirus.

Article Snippet: 1547 , DPP4 (clone OTI11D7) , MAB , 1:100 , PK , Origene Technologies , (no. 1371) Envision Doublestain , .

Techniques: Immunohistochemical staining, Staining, Infection, Membrane

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Double immunofluorescent staining of human subcutaneous adipose tissue sections with antibodies against T-cadherin (green) and DPP4 (red); nuclei were counterstained with DAPI (blue). Images were acquired using a Zeiss LSM 780 confocal microscope and ZEN2010 software, shown at lower magnification (A) and higher magnification (B) . A thick arrow points to a group of cells expressing both T-cadherin and DPP4 in the interstitium; thin arrows mark cells expressing only T-cadherin; ovals encircle adipocytes. Scale bar 50 µm. (C) The table shows the percentage of T-cadherin–positive, DPP4 + cells and double-positive cells (DPP4 + /T-cadherin + ), quantified from adipose tissue sections of two healthy donors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Staining, Microscopy, Software, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Light microscopy of MSCs (of the two to three passages) isolated from human subcutaneous adipose tissue of a healthy donor (A) and immunofluorescent staining with antibodies against T-cadherin (green) (B) . Arrows indicate cells with low or no T-cadherin expression, whereas cells exhibiting green fluorescence corresponding to T-cadherin are clearly visible. Scale bar, 50 µm. Light microscopy of human MSCs (C) and double immunofluorescent staining with antibodies against T-cadherin green, (E) and DPP4 red, (F) nuclei were counterstained with DAPI blue, (D) . Arrows in (C–F) indicate one and the same cell co-expressing T-cadherin and DPP4. Images were acquired using a Leica DMI 6000B microscope equipped with a Leica DFC7000T digital camera and LAS X software. Scale bar, 20 µm. (G) Representative flow cytometry plot showing T-cadherin and DPP4 distribution in cultured MSCs. The proportion of double-positive (DPP4 + /T-cadherin + ) cells was 30.4%; 6.15% expressed only T-cadherin, and 14% expressed only DPP4.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Light Microscopy, Isolation, Staining, Expressing, Fluorescence, Microscopy, Software, Flow Cytometry, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Individual UMAP plots showing the expression levels and distribution of CDH13 (encoding T-cadherin) in control MSCs (A) and MSCs after 4 days of adipogenic induction (B) . UMAP plots demonstrating DPP4 expression in control MSCs (C) and MSCs after 4 days of adipogenic induction (D) . (E) RT-qPCR analysis of MSCs cultured in control medium or under adipogenic induction conditions showing the dynamics of T-cadherin mRNA expression. T-cadherin/ CDH13 expression decreased by day 4 in adipogenic medium and remained low through day 10. RT-qPCR data are shown as the mean ± SD. T-test. **р< 0.01 *p < 0.05 vs. control media in corresponding experimental day. Results are representative of three biologically independent experiments.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Quantitative RT-PCR, Cell Culture

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. (A) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression (encoding for T-cadherin) in the integrated object; CDH13 expressing cells corresponds to Cluster 3 (more than 1-fold change of the average expression level); (B) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the integrated object; DPP4 expressing cells correspond to Cluster 3 (more than 1-fold change of the average expression level) (C) DimPlot–Integrated object UMAP-clustering. Sample proportion diagrams depict the ratio between the cell counts in the control MSC sample (Salmon) and in the MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation within the Clusters. (D) DimPlot–Integrated object grouped by samples. CDH13 expression in the control MSC sample (Salmon) and MSC sample (Iris blue) after a 4-day induction of adipogenic differentiation. Cluster 3 predominantly contains cells from the control sample.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Integrated object. FeaturePlot. Each cluster is denoted by color. Cluster 0 (Salmon) primarily contains cells expressing fibroblast markers and genes responsible for cell cycle regulation. Cluster 1 (Khaki) encompasses cells expressing preadipocyte-specific genes, such as CEBPB , PPARγ, CD36 and markers of mature adipocytes ( ADIPOQ , Perilipin1 , Perilipin4 ). In Cluster 2 (green), cells predominantly express genes related to mitosis. Cluster 3 (Blue) contains cells of interest with high level of T-cadherin expression, as well as classical MSC markers ( CD90 , PDGFR ), Wnt signaling genes , and DPP4 . In a separate remote Cluster 4 (Magenta), besides CDH13 , cells express Nestin , a marker of neural crest cells, and CD36 , a marker of adipocyte progenitors.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Split violin-plots showing the relative expression levels and distribution of CDH13 (A) and DPP4 (B) genes in the control MSC sample (Salmon) and MSC sample after a 4-day induction of adipogenic differentiation (Iris blue). The highest CDH13 expression was detected in Cluster 3 in MSCs of the control sample compared to MSCs after a 4-day adipogenic induction. Similarly, the highest expression of DPP4 was found in Cluster 3 in MSCs of the control sample. Split violin plots were generated using the R package Seurat and the function VlnPlot with the argument split.by = “sample”.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Control, Generated

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: (A) DimPlot– GSE182158 object UMAP-clustering; (B) 2 cluster manual cell type annotation, the red oval marks cluster 2; (C) FeaturePlot–UMAP-plot showing principal distribution of CDH13 gene expression in the GSE182158 object; (D) FeaturePlot–UMAP-plot showing principal distribution of DPP4 gene expression (encoding for T-cadherin) in the GSE182158 object.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: Adiponectin receptor T-cadherin emerges as a novel regulator of adipose stem cell quiescence and adipogenesis

doi: 10.3389/fcell.2025.1734183

Figure Lengend Snippet: Elevated DPP4 expression in MSCs after lentiviral transduction in T-cadherin-overexpressing cells was verified using RT-qPCR (A) and Western blot (B) . β-tubulin was used as the loading control for Western blot analysis. Representative results from one of two biologically independent RT-qPCR and eight Western blot experiments are shown. ANOVA with multiple comparisons, **p < 0.01.

Article Snippet: Cells were detached from culture dishes using HyQTase Detachment Reagent (HyClone, GE Healthcare Life Sciences, United States) and stained with appropriate combinations of primary antibodies against: DPP4 (CD26 Antibody (MA2607), ThermoFisher Scientific, dilution 1:100), T-cadherin (ProSci, United States, #3583, dilution 1:100).

Techniques: Expressing, Transduction, Quantitative RT-PCR, Western Blot, Control

Journal: Biomedicines

Article Title: Is Spheroid a Relevant Model to Address Fibrogenesis in Keloid Research?

doi: 10.3390/biomedicines11092350

Figure Lengend Snippet: Figure 4. Three-Dimensional culture and TGF-β1 activation converge to downregulate CD26 and TGFβRII expression. TGFβRII (A) and DPP4 (B) mRNA synthesis were evaluated by RT-qPCR in monolayers and spheroids performed either with NDFs or KFs in the presence of TGF-β1 vs. control [n = 4 per condition]. After 7 days of treatment, spheroids thus treated were immunostained for CD26 (green) and TGFβRII (red) observation (C) and semi quantification (D,E). Nuclei were counterstained with DAPI (Blue). [n = 10 spheroid and n= 2–6 images per spheroid]. Statistical analyses were performed using two-way ANOVA * for p < 0.05; ** for p < 0.005; *** for p < 0.001; **** for p < 0.0001.

Article Snippet: Appropriate dilutions of primary antibodies targeting αSMA (A2547, Sigma Aldrich, Saint-Louis, MO, USA), CD26 (OTI11D7, OriGene, Rockville, MD, USA), and TGFβRII (PA5-35076, Invitrogen, Carlsbad, CA, USA) were added on glass slides before incubation (overnight, 4 ◦C in a wet chamber).

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Control